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雑種致死

異なる植物間で交雑を行うと、雑種胚や雑種植物が致死することがあります。これを雑種致死と呼びます。当研究室では特に雑種植物に生じる致死性に注目して研究を行っています。

タバコ雑種致死の様子.jpg

タバコ種間雑種における雑種致死の様子（Tezuka 2013）

雑種致死は遺伝的に制御された現象であり、交雑組合せによって雑種致死が生じるか、生じないかが決まっています。これまでに、雑種致死はタバコの生育に適した温度で生じますが、雑種を高温で栽培することで回避できることがわかっています。

温度感受性28-36度.jpg

高温条件による雑種致死の回避（左から28、30、32、34、36℃）（Tezuka et al. 2007）

遺伝学や分子生物学的な手法を駆使した実験により、雑種致死の機構がある程度わかってきていますが、まだまだ未解明の点が多く残されています。引き続き、雑種致死が生じる機構などを明らかにするために研究を行っています。

雑種致死に関連する発表論文

*：責任著者、下線：本研究グループの学生など、太字下線：教員

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  1. Masanobu Mino*, Takahiro Tezuka, Sachiko Shomura (2022) The hybrid lethality of interspecific F1 hybrids of Nicotiana: a clue to understanding hybrid inviability—a major obstacle to wide hybridization and introgression breeding of plants. Molecular Breeding 42(2): 10

  2. Takahiro Tezuka*, Naoto Kitamura, Masanori Yanase, Toshinobu Morikawa (2021) Evaluation of crossability between Nicotiana benthamiana and Nicotiana excelsior. Agronomy 11(12): 2583

  3. Takahiro Tezuka*, Naoto Kitamura, Sae Imagawa, Akira Hasegawa, Kumpei Shiragaki, Hai He, Masanori Yanase, Yoshiyuki Ogata, Toshinobu Morikawa, Shuji Yokoi (2021) Genetic mapping of the HLA1 locus causing hybrid lethality in Nicotiana interspecific hybrids. Plants 10(10): 2062

  4. Kenji Kawaguchi, Yuichiro Ohya, Maho Maekawa, Takahiro Iizuka, Akira Hasegawa, Kumpei Shiragaki, Hai He, Masayuki Oda, Toshinobu Morikawa, Shuji Yokoi, Takahiro Tezuka* (2021) Two Nicotiana occidentalis accessions enable gene identification for Type II hybrid lethality by the cross to N. sylvestris. Scientific Reports 11: 17093

  5. Yushi Katsuyama, Mizuho Doi, Sachi Shioya, Sanae Hane, Momoko Yoshioka, Shuichi Date, Chika Miyahara, Tomomichi Ogawa, Ryo Takada, Hanako Okumura, Rie Ikusawa, Sakihito Kitajima, Kenji Oda, Kenji Sato, Yoshikazu Tanaka, Takahiro Tezuka, Masanobu Mino* (2021) The role of chaperone complex HSP90-SGT1-RAR1 as the associated machinery for hybrid inviability between Nicotiana gossei Domin and N. tabacum L. Gene 776:145443

  6. Kumpei Shiragaki, Rie Nakamura, Shigeki Nomura, Hai He, Tetsuya Yamada, Wataru Marubashi, Masayuki Oda, Takahiro Tezuka* (2020) Phenylalanine ammonia-lyase and phenolic compounds are related to hybrid lethality in the cross Nicotiana suaveolens × N. tabacum. Plant Biotechnology 37(3): 327-333

  7. Hai He, Takahiro Iizuka, Maho Maekawa, Kumi Sadahisa, Toshinobu Morikawa, Masanori Yanase, Shuji Yokoi, Masayuki Oda, Takahiro Tezuka* (2019) Nicotiana suaveolens accessions with different ploidy levels exhibit different reproductive isolation mechanisms in interspecific crosses with Nicotiana tabacum. Journal of Plant Research 132(4): 461-471

  8. Takahiro Iizuka, Tsutomu Kuboyama, Wataru Marubashi, Masayuki Oda, Takahiro Tezuka* (2012) Nicotiana debneyi has a single dominant gene causing hybrid lethality in crosses with N. tabacum. Euphytica 186(2): 321-328

  9. Takahiro Tezuka*, Chihiro Matsuo, Takahiro Iizuka, Masayuki Oda, Wataru Marubashi (2012) Identification of Nicotiana tabacum linkage group corresponding to the Q chromosome gene(s) involved in hybrid lethality. PLoS ONE 7(5): e37822

  10. Takahiro Tezuka*, Wataru Marubashi (2012) Genes in S and T subgenomes are responsible for hybrid lethality in interspecific hybrids between Nicotiana tabacum and Nicotiana occidentalis. PLoS ONE 7(4): e36204

  11. 手塚孝弘*・丸橋亘（2011）タバコ栽培種とSuaveolentes節に属するタバコ野生種との種間雑種に認められる雑種致死．作物研究 56: 9-15

  12. Takahiro Tezuka*, Tsutomu Kuboyama, Toshiaki Matsuda, Wataru Marubashi (2010) Seven of eight species in Nicotiana section Suaveolentes have common factors leading to hybrid lethality in crosses with Nicotiana tabacum. Annals of Botany 106(2): 267-276

  13. Takahiro Tezuka, Tsutomu Kuboyama, Toshiaki Matsuda, Wataru Marubashi* (2007) Possible involvement of genes on the Q chromosome of Nicotiana tabacum in expression of hybrid lethality and programmed cell death during interspecific hybridization to Nicotiana debneyi. Planta 226(3): 753-764

  14. Takahiro Tezuka, Wataru Marubashi* (2006) Genomic factors lead to programmed cell death during hybrid lethality in interspecific hybrids between Nicotiana tabacum and N. debneyi. SABRAO Journal of Breeding and Genetics 38(2): 69-81

  15. Takahiro Tezuka, Wataru Marubashi* (2006) Hybrid lethality in interspecific hybrids between Nicotiana tabacum and N. suaveolens: evidence that the Q chromosome causes hybrid lethality based on Q-chromosome-specific DNA markers. Theoretical and Applied Genetics 112(6): 1172-1178

  16. Takahiro Tezuka, Katsura Onosato, Saori Hijishita, Wataru Marubashi* (2004) Development of Q-chromosome-specific DNA markers in tobacco and their use for identification of a tobacco monosomic line. Plant and Cell Physiology 45(12): 1863-1869

  17. Takahiro Tezuka, Wataru Marubashi* (2004) Apoptotic cell death observed during the expression of hybrid lethality in interspecific hybrids between Nicotiana tabacum and N. suaveolens. Breeding Science 54(1): 59-66

雑種致死に関連する発表著書

  1. Takahiro Tezuka* (2013) Hybrid lethality in Nicotiana: a review with special attention to interspecific crosses between species in sect. Suaveolentes and N. tabacum. In: Florian Wallner (ed) Herbaceous Plants: Cultivation Methods, Grazing and Environmental Impacts. Nova Science Publishers, New York, pp. 69-94

  2. Takahiro Tezuka* (2012) Hybrid lethality in the genus Nicotiana. In: John Kiogora Mworia (ed) Botany. InTech, Rijeka, Croatia, pp. 191-210

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